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5

SEMI PREPARATIVE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR SMALL SCALE
SEPARATION AND ISOLATION 

(LC PREP)

NoDetails
1Location:
Advanced Mass Spectrometry Laboratory (01-59-02)
2Details of Instrument:

The JAI Recycling HPLC, LC 9110II Next is a High Performance Liquid Chromatography with Recycling function for purification and isolation for separation of structurally very close of compounds and getting certain peaks from organic synthesis and natural products. The system/software have panel to control the pump, injector, detector and fraction collector from computer or LCD touch screen panel.

This system use UV detector is capable as simultaneously able to detect at 4 selected wavelength from 200-800nm. For further information this system as below:






















































Desription Minimum Maximum
Wavelength range 200nm 800nm
Sample weight (dillute  to  solvent 1-3mL) 100mg 250mg
Pressure 1.00MPa 7.00Mpa
Flowrate 0.5ml/min 7.5ml/min
Solvent – Chloroform only, for dillution sample 1.0 mL 3.0mL
Mobile phase- Chloroform only 400 mL 1800mL
Size of sample bottle 20mL -
Quantity of sample bottle 10 units 20 units
Fraction of collector 1 33


The system with the 20mmID column and 600mm length able to work with organic solvent of chloroform for four columns. The types and exclusion limits of columns as followed.































Desription Exlusion Limits Average of Molecular Weight
JAIGEL – 1HH 1000 500
JAIGEL – 2HH 5000 2500
JAIGEL – 2.5 HH 2X104 10,000
JAIGEL – 3HH 7X104 35,000
3Service ChargePlease refer to P.I.C for quotation.
4Operation HoursSunday - Thursday

9.00 am - 4.00 pm
5StatusAvailable for booking
6P.I.CNormah Kasimon
normahk@utm.my
07-5557718

Malahah Mohamed
malahah@utm.my
07-5557718
7Download Booking FormClick Here
Updated on : 15/01/2018

 

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